Pax3^{tm2.1(PAX3/FOXO1A)Buck}
Targeted Allele Detail

Summary

• Symbol: Pax3^{tm2.1(PAX3/FOXO1A)Buck}
• Name: paired box 3; targeted mutation 2.1, Margaret E Buckingham
• MGI ID: MGI:2687299
• Synonyms: Pax3^PAX3-FKHR, Pax3^{PAX3-FKHR-IRESnLacZ}
• Gene: Pax3 Location: Chr1:78077904-78173771 bp, - strand Genetic Position: Chr1, 39.79 cM
• Alliance: Pax3^{tm2.1(PAX3/FOXO1A)Buck} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86911
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The floxed puromycin cassette was removed from Pax3^{tm2(PAX3/FOXO1A)Buck} via cre mediated recombination, allowing for the expression of sequence encoding a human PAX3/FOXO1A fusion as well as lacZ. (J:86911)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pax3 Mutation: 50 strains or lines available

Notes

This allele was generated in J:86911 using a cross between females carrying Tg(Pgk1-cre)1Lni and males carrying the floxed allele. As this cre transgene is expressed in diploid eggs, all offspring are exposed to Cre protien, independent of inheritance of the cre transgene.

References

• Original: J:86911 Relaix F, et al., The transcriptional activator PAX3-FKHR rescues the defects of Pax3 mutant mice but induces a myogenic gain-of-function phenotype with ligand-independent activation of Met signaling in vivo. Genes Dev. 2003 Dec 1;17(23):2950-65
• All: 8 reference(s)