Rorc^tm2Litt
Targeted Allele Detail

Summary

• Symbol: Rorc^tm2Litt
• Name: RAR-related orphan receptor gamma; targeted mutation 2, Dan R Littman
• MGI ID: MGI:3026635
• Synonyms: Roc(gt)^gfp, Rorc(gammat)^-, Rorc(gammat)-EGFP, Rorc^GFP, RORgammat, RORgammat-EGFP
• Gene: Rorc Location: Chr3:94280106-94305583 bp, + strand Genetic Position: Chr3, 40.56 cM, cytoband F2
• Alliance: Rorc^tm2Litt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87395
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An isoform-specific disruption was generated by inserting a cassette containing eGFP downstream of the start codon of exon 1gammat. The targeted exon is the third exon of the gene, serves as an alternative first exon for the gammat transcript, and is not included in the gamma transcript. RT-PCR analysis of hepatic RNA obtained from homozygous mutant mice identified gamma transcript but not the targted gammat transcript. The eGFP sequence included a stop codon but no splice sites or polyadenylation signals. A floxed neo cassette included in the targeting vector was excised from the germline via cre-mediated recombination. (J:87395)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Rorc Mutation: 44 strains or lines available

References

• Original: J:87395 Eberl G, et al., An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells. Nat Immunol. 2004 Jan;5(1):64-73
• All: 182 reference(s)