Efnb3^tm1Henk
Targeted Allele Detail

Summary

• Symbol: Efnb3^tm1Henk
• Name: ephrin B3; targeted mutation 1, Mark Henkemeyer
• MGI ID: MGI:3026700
• Synonyms: ephrinB3^KO, ephrin-B3^neo
• Gene: Efnb3 Location: Chr11:69444918-69451031 bp, - strand Genetic Position: Chr11, 42.8 cM
• Alliance: Efnb3^tm1Henk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87497
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An frt-flanked neo cassette and a loxP-flanked lacZ cassette were inserted at the endogenous locus. The neo cassette was inserted at a restriction site within intron 4 while the lacZ cassette was inserted in frame in exon 5, disrupting codons 264 through 340 which make up the majority of the cytoplasmic domain. Normal protein was undetected in homozygous mutant mice by Northern blot analysis, however a truncated transcript was detected. This mutation is expected to ablate both forward and reverse signaling. The presence of the neo cassette prevented expression of lacZ. (J:87497)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Efnb3 Mutation: 11 strains or lines available

References

• Original: J:87497 Yokoyama N, et al., Forward signaling mediated by ephrin-B3 prevents contralateral corticospinal axons from recrossing the spinal cord midline. Neuron. 2001 Jan;29(1):85-97
• All: 16 reference(s)