Efnb3^tm1.1Henk
Targeted Allele Detail

Summary

• Symbol: Efnb3^tm1.1Henk
• Name: ephrin B3; targeted mutation 1.1, Mark Henkemeyer
• MGI ID: MGI:3026702
• Synonyms: ephrin3^lacZ, ephrin-B3^lacZ
• Gene: Efnb3 Location: Chr11:69444918-69451031 bp, - strand Genetic Position: Chr11, 42.8 cM
• Alliance: Efnb3^tm1.1Henk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87497
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The frt-flanked neo cassette was excised from Efnb2^tm1Henk via FLP-mediated recombination, leaving the loxP flanked lacZ cassette in exon 5. The lacZ cassette disrupted codons 264 through 340 which make up the majority of the cytoplasmic domain. Biochemical analysis indicated that the expected EFNB2-beta-gal protein, in which the endogenous cytoplasmic domain is replaced with beta-gal, was produced. This mutation leaves forward signaling intact and ablates reverse signaling. The expression pattern of lacZ was observed to be to similar to that of the endogenous gene. (J:87497)

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Efnb3 Mutation: 11 strains or lines available

References

• Original: J:87497 Yokoyama N, et al., Forward signaling mediated by ephrin-B3 prevents contralateral corticospinal axons from recrossing the spinal cord midline. Neuron. 2001 Jan;29(1):85-97
• All: 17 reference(s)