Atp1a2^tm3Ling
Targeted Allele Detail

Summary

• Symbol: Atp1a2^tm3Ling
• Name: ATPase, Na+/K+ transporting, alpha 2 polypeptide; targeted mutation 3, Jerry B Lingrel
• MGI ID: MGI:3027260
• Synonyms: alpha2^R
• Gene: Atp1a2 Location: Chr1:172099276-172125631 bp, - strand Genetic Position: Chr1, 79.6 cM
• Alliance: Atp1a2^tm3Ling page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87082
• Parent Cell Line: Duffy (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: Two missense mutations (L111R and N122D) were engineered into exon 4. A floxed neo cassette included in the targeting vector was excised from intron 3 prior to germline transmission. The mutations, which manifest in the first extracellular domain of the alpha 2 isoform, impair cardiac glycoside affinity. Western blot analysis of mutant mice indicated that the mutations did not affect either the level protein expression or the distribution of the alpha 1 and alpha 3 isoforms. (J:87082)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Atp1a2 Mutation: 62 strains or lines available

References

• Original: J:87082 Dostanic I, et al., The alpha2 isoform of Na,K-ATPase mediates ouabain-induced cardiac inotropy in mice. J Biol Chem. 2003 Dec 26;278(52):53026-34
• All: 18 reference(s)