Spo11^tm1Rdco
Targeted Allele Detail

Summary

• Symbol: Spo11^tm1Rdco
• Name: SPO11 initiator of meiotic double stranded breaks; targeted mutation 1, R Daniel Camerini-Otero
• MGI ID: MGI:3027823
• Synonyms: Spo11^-
• Gene: Spo11 Location: Chr2:172819493-172835369 bp, + strand Genetic Position: Chr2, 95.64 cM, cytoband H4
• Alliance: Spo11^tm1Rdco page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:65881
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 starting after the sequence coding for the 3rd amino acid after the second "in frame" ATG through nucleotide 35 of the first intron was replaced with a PGK-neomycin cassette in reverse orientation. This removes the splice donor site. Absence of transcript was confirmed by Northern blot analysis. (J:65881)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spo11 Mutation: 21 strains or lines available

References

• Original: J:65881 Romanienko PJ, et al., The mouse spo11 gene is required for meiotic chromosome synapsis. Mol Cell. 2000 Nov;6(5):975-87
• All: 19 reference(s)