Dsc1^tm1Pko
Targeted Allele Detail

Summary

• Symbol: Dsc1^tm1Pko
• Name: desmocollin 1; targeted mutation 1, Peter J Koch
• MGI ID: MGI:3028701
• Synonyms: dsc1^deltaE17LoxP, dsc1^{-deltaE17LoxP}
• Gene: Dsc1 Location: Chr18:20217241-20247928 bp, - strand Genetic Position: Chr18, 11.17 cM
• Alliance: Dsc1^tm1Pko page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87593
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Intron 16 and exon 17 were deleted and the 3' end of exon 16 was fused to the 3' UTR. A floxed PGK-neomycin cassette was also inserted 0.23kb downstrean of exon 16. The neomycin cassette was subsequently excised by transient transfection of properly targeted ES cells with a Cre expression plasmid. RT-PCR analysis indicated that an aberrant mRNA was produced which retained intron 15. A truncated protein product is expected rather than gene disruption. (J:87593)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dsc1 Mutation: 49 strains or lines available

References

• Original: J:87593 Cheng X, et al., Assessment of splice variant-specific functions of desmocollin 1 in the skin. Mol Cell Biol. 2004 Jan;24(1):154-63
• All: 1 reference(s)