Pou2f1^tm1Shrp
Targeted Allele Detail

Summary

• Symbol: Pou2f1^tm1Shrp
• Name: POU domain, class 2, transcription factor 1; targeted mutation 1, Phillip A Sharp
• MGI ID: MGI:3028868
• Synonyms: Oct-1^-
• Gene: Pou2f1 Location: Chr1:165692723-165830247 bp, - strand Genetic Position: Chr1, 73.21 cM
• Alliance: Pou2f1^tm1Shrp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87664
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 was replaced with a floxed neo cassette. The deleted exon is common to all three known isoforms and does not overlap with Cd247, a locus that overlaps a distal portion of the Pou2f1 locus. Mutant transcript, in which exon 2 aberrantly splices to exon 4a, was identified by RT-PCR analysis of homozygous mutant MEFs. The aberrant splicing results in a frameshift mutation which consequently generates a nonsense mutation and precludes the translation of two-thirds of the mature protein including the DNA-binding domain. Supershift analysis of DNA binding assays using nuclear extracts from homozygous cells detected very low levels of protien, thus this is a severely hypomorphic allele. (J:87664)

Expression

In Mice Carrying this Mutation:	34 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Pou2f1^tm1Shrp

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pou2f1 Mutation: 99 strains or lines available

References

• Original: J:87664 Wang VE, et al., Embryonic lethality, decreased erythropoiesis, and defective octamer-dependent promoter activation in Oct-1-deficient mice. Mol Cell Biol. 2004 Feb;24(3):1022-32
• All: 7 reference(s)