Grb14^tm1Daly
Targeted Allele Detail

Summary

• Symbol: Grb14^tm1Daly
• Name: growth factor receptor bound protein 14; targeted mutation 1, Roger J Daly
• MGI ID: MGI:3029164
• Synonyms: Grb14^-
• Gene: Grb14 Location: Chr2:64742820-64853143 bp, - strand Genetic Position: Chr2, 38.35 cM
• Alliance: Grb14^tm1Daly page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87892
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The endogenous locus was disrupted a cassette encoding lacZ and GFP inserted downstream of the start codon via homologous recombination. A floxed neo cassette was also included in the targeting vector, but was excised via cre-mediated recombination in the germline. Neither normal transcript nor protein was detected by Northern and Western blot analyses of homozygous mutant mice. LacZ expression was realized via anti-beta-gal staining. (J:87892)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grb14 Mutation: 29 strains or lines available

References

• Original: J:87892 Cooney GJ, et al., Improved glucose homeostasis and enhanced insulin signalling in Grb14-deficient mice. EMBO J. 2004 Feb 11;23(3):582-93
• All: 8 reference(s)