Ltc4s^tm1Blam
Targeted Allele Detail

Summary

• Symbol: Ltc4s^tm1Blam
• Name: leukotriene C4 synthase; targeted mutation 1, Bing K Lam
• MGI ID: MGI:3029254
• Synonyms: LTC4S^-
• Gene: Ltc4s Location: Chr11:50127288-50129378 bp, - strand Genetic Position: Chr11, 30.4 cM, cytoband B1.1-B1.2
• Alliance: Ltc4s^tm1Blam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87909
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin gene cassette replaced 289 nucleotides from intron 1 as well as exons 2 through 4 and 32 nucleotides from intron 4. Northern blot analysis of RNA from bone marrow macrophages confirmed the absence of normal transcripts. An exon 5 containing transcript was produced however. No leukotriene C4 was detectable. (J:87909)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ltc4s Mutation: 15 strains or lines available

References

• Original: J:87909 Kanaoka Y, et al., Attenuated zymosan-induced peritoneal vascular permeability and IgE-dependent passive cutaneous anaphylaxis in mice lacking leukotriene C4 synthase. J Biol Chem. 2001 Jun 22;276(25):22608-13
• All: 25 reference(s)