Bad^tm1Meg
Targeted Allele Detail

Summary

• Symbol: Bad^tm1Meg
• Name: BCL2-associated agonist of cell death; targeted mutation 1, Michael E Greenberg
• MGI ID: MGI:3029528
• Synonyms: BAD^3SA
• Gene: Bad Location: Chr19:6919229-6929267 bp, + strand Genetic Position: Chr19, 5.1 cM
• Alliance: Bad^tm1Meg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88139
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The sequences coding for the three regulatory serines: Ser-112, Ser-136, and Ser-155 were all altered to code for alanines while an FRT flanked PGK-neomycin resistance cassette was inserted into the 3' UTR. The neomycin gene was subsequently excised by crossing mutant mice to transgenic mice that express FlpE recombinase. Western blot analysis demonstrated that the level of protein expression was similar to that of the wild-type protein but that the protein produced could no longer be phosphorylated at the three regulatory serines. (J:88139)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bad Mutation: 25 strains or lines available

References

• Original: J:88139 Datta SR, et al., Survival factor-mediated BAD phosphorylation raises the mitochondrial threshold for apoptosis. Dev Cell. 2002 Nov;3(5):631-43
• All: 8 reference(s)