Piwil2^tm1Nkn
Targeted Allele Detail

Summary

• Symbol: Piwil2^tm1Nkn
• Name: piwi-like RNA-mediated gene silencing 2; targeted mutation 1, Toru Nakano
• MGI ID: MGI:3029596
• Synonyms: Mili^-
• Gene: Piwil2 Location: Chr14:70609926-70666832 bp, - strand Genetic Position: Chr14, 36.32 cM
• Alliance: Piwil2^tm1Nkn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87735
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 5.2 kb genomic fragment extending from the start codon of exon 2 through exon 5 was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded residues 1 through 209. Protein was undetected in homozygous mutant testes by Western blot analysis. (J:87735)

Expression

In Mice Carrying this Mutation:	45 assay results 4 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Piwil2 Mutation: 54 strains or lines available

References

• Original: J:87735 Kuramochi-Miyagawa S, et al., Mili, a mammalian member of piwi family gene, is essential for spermatogenesis. Development. 2004 Feb;131(4):839-49
• All: 22 reference(s)