Anapc2^tm1Kna
Targeted Allele Detail

Summary

• Symbol: Anapc2^tm1Kna
• Name: anaphase promoting complex subunit 2; targeted mutation 1, Kim Nasmyth
• MGI ID: MGI:3029825
• Synonyms: Apc2^flox
• Gene: Anapc2 Location: Chr2:25162490-25175927 bp, + strand Genetic Position: Chr2, 17.12 cM
• Alliance: Anapc2^tm1Kna page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87782
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted in intron 4 while a floxed and Frt flanked neomycin-Tk selection cassette was inserted in intron 1. Transient transfection with a Flpc-recombinase expressing plasmid results in excision of the neomycin-Tk cassette and leaves exons 2-4 flanked by loxP sites. (J:87782)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Anapc2 Mutation: 36 strains or lines available

References

• Original: J:87782 Wirth KG, et al., Loss of the anaphase-promoting complex in quiescent cells causes unscheduled hepatocyte proliferation. Genes Dev. 2004 Jan 1;18(1):88-98
• All: 4 reference(s)