Runx1^tm1Toku
Targeted Allele Detail

Summary

• Symbol: Runx1^tm1Toku
• Name: runt related transcription factor 1; targeted mutation 1, Tsukasa Okuda
• MGI ID: MGI:3029984
• Synonyms: AML1^Delta446, Runx1^delta446, Runx1^deltaV
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^tm1Toku page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87697
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Hypomorph, Inserted expressed sequence)
• Mutation: Other
• Mutation details: A cDNA encoding a C-terminally truncated RUNX1 protein was inserted into exon 4 of the endogenous locus via homologous recombination. The encoded protein truncates at residue 446 and thereby retains the trans-activating subdomains and lacks the VWRPY motif. Semi-quantitative RT-PCR analysis of splenic and thymic message showed the levels of mutant transcript in homozygous mutant mice to be similar to the levels of normal transcript in wild-type mice. (J:87697)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 34 strains or lines available

References

• Original: J:87697 Nishimura M, et al., VWRPY motif-dependent and -independent roles of AML1/Runx1 transcription factor in murine hematopoietic development. Blood. 2004 Jan 15;103(2):562-70
• All: 9 reference(s)