Trp53^tm2Glo
Targeted Allele Detail

Summary

• Symbol: Trp53^tm2Glo
• Name: transformation related protein 53; targeted mutation 2, Guillermina Lozano
• MGI ID: MGI:3032598
• Synonyms: p53^C, p53^P, pP53-R172P, Trp53^515C
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm2Glo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87501
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Single point mutation
• Mutation details: An arginine to proline substitution was generated at codon 172 by an engineered point mutation introduced at coding nucleotide 515 (c.515G>C). p.R172P, equivalent to p.R175P in humans, is associated with human tumors defective in apoptosis. A single loxP site was left in intron 4 after the excision of a loxP site flanked neomycin resistance gene cassette via Cre-mediated recombination. Western blot analysis of homozygous MEFs detected full length protein at levels that exceeded those of full length protein in wild-type MEFs. (J:87501)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Trp53^tm2Glo

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 240 strains or lines available

References

• Original: J:87501 Liu G, et al., Chromosome stability, in the absence of apoptosis, is critical for suppression of tumorigenesis in Trp53 mutant mice. Nat Genet. 2004 Jan;36(1):63-68
• All: 18 reference(s)