Actb^{tm1(PyVT)Arge}
Targeted Allele Detail

Summary

• Symbol: Actb^{tm1(PyVT)Arge}
• Name: actin, beta; targeted mutation 1, Argiris Efstratiadis
• MGI ID: MGI:3032612
• Synonyms: Beta-actin^PVMT
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^{tm1(PyVT)Arge} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87544
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A targeting construct was designed to insert sequence encoding IRES, floxed neo, and polyomavirus middle T antigen (PyVT) in the 3' untranslated region of beta actin, 55 bp downstream of the stop codon. The inserted cassette leaves the endogenous coding region and the 54 bp 'zipcode' that directs cellular localization of beta actin intact. PyVT expression is dependent on the removal of the floxed neo cassette (which occurred at low frequency without cre expression). (J:87544)

Tumor Data

List all tumor models in MMHCdb carrying Actb^{tm1(PyVT)Arge}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 53 strains or lines available

References

• Original: J:87544 Politi K, et al., 'Designer' tumors in mice. Oncogene. 2004 Feb 26;23(8):1558-65
• All: 1 reference(s)