Gli2^{tm5.1(GLI3)Alj}
Targeted Allele Detail

Summary

• Symbol: Gli2^{tm5.1(GLI3)Alj}
• Name: GLI-Kruppel family member GLI2; targeted mutation 5.1, Alexandra L Joyner
• MGI ID: MGI:3032719
• Synonyms: Gli2^3ki
• Gene: Gli2 Location: Chr1:118761862-118981349 bp, - strand Genetic Position: Chr1, 52.17 cM, cytoband E2-E4
• Alliance: Gli2^{tm5.1(GLI3)Alj} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87948
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Gli2^{tm5.1(GLI3)Alj} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  GLI3 (2737)

  Gli3	(MGI:95729)

• Mutation details: The floxed neo cassette was excised from Gli2^tm5(GLI3)Alj, leaving the human GLI3 cDNA, flanked with an upstream FLAG epitope and a downstream mouse Gli2 3' UTR and three tandem repeats of a SV40 polyA signal, positioned within the first coding exon of the endogenous Gli2 locus. Western blot analysis of mutant mice indicated that the full length and the repressor form of the GLI3 protein were present. The level of GLI3 expression from this allele was reported to be greater than from Gli2^tm5(GLI3)Alj. (J:87948)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gli2 Mutation: 169 strains or lines available

References

• Original: J:87948 Bai CB, et al., All mouse ventral spinal cord patterning by hedgehog is Gli dependent and involves an activator function of Gli3. Dev Cell. 2004 Jan;6(1):103-15
• All: 1 reference(s)