Gsta4^tm1Pzim
Targeted Allele Detail

Summary

• Symbol: Gsta4^tm1Pzim
• Name: glutathione S-transferase, alpha 4; targeted mutation 1, Piotr Zimniak
• MGI ID: MGI:3032766
• Gene: Gsta4 Location: Chr9:78099248-78116631 bp, + strand Genetic Position: Chr9, 43.65 cM, cytoband E1
• Alliance: Gsta4^tm1Pzim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87983
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A sequence fragment encompassing the majority of exon 4 and a portion of the following intron was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded amino acids 60 through 91, which have been previously implicated in protein structure and dimerization. Northern and Western blots of a variety of tissues indicated an absence of both transcript and protein in homozygous mutant mice. (J:87983)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gsta4 Mutation: 65 strains or lines available

References

• Original: J:87983 Engle MR, et al., Physiological role of mGSTA4-4, a glutathione S-transferase metabolizing 4-hydroxynonenal: generation and analysis of mGsta4 null mouse. Toxicol Appl Pharmacol. 2004 Feb 1;194(3):296-308
• All: 12 reference(s)