Dph1^tm1Bhr
Targeted Allele Detail

Summary

• Symbol: Dph1^tm1Bhr
• Name: diphthamide biosynthesis 1; targeted mutation 1, Richard R Behringer
• MGI ID: MGI:3033421
• Synonyms: Ovca1 ^-
• Gene: Dph1 Location: Chr11:75068469-75081309 bp, - strand Genetic Position: Chr11, 45.76 cM
• Alliance: Dph1^tm1Bhr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88090
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A linker containing stop codons in all three reading frames together with a floxed neomycin resistance cassette were introduced into exon 3. Targeting took place in ES cells homozygous for the Tg(Prm-cre)70Og transgene. Cre-mediated excision subsequently removed the neomycin cassette upon transmission from male chimeric mice. mRNA transcripts were produced but Western blot analysis demonstrated the absence of protein. (J:88090)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Dph1^tm1Bhr

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dph1 Mutation: 27 strains or lines available

References

• Original: J:88090 Chen CM, et al., Ovca1 regulates cell proliferation, embryonic development, and tumorigenesis. Genes Dev. 2004 Feb 1;18(3):320-32
• All: 3 reference(s)