Dph1^tm2Bhr
Targeted Allele Detail

Summary

• Symbol: Dph1^tm2Bhr
• Name: diphthamide biosynthesis 1; targeted mutation 2, Richard R Behringer
• MGI ID: MGI:3033425
• Synonyms: Ovca1-2^-
• Gene: Dph1 Location: Chr11:75068469-75081309 bp, - strand Genetic Position: Chr11, 45.76 cM
• Alliance: Dph1^tm2Bhr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88090
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 7 to 12 as well as part of exon 13 were replaced with a floxed neomycin resistance gene. In the process, exon 1 of Ovca2 which is located between exons 12 and 13 was also deleted. Targeting took place in PC3 ES cells homozygous for Tg(Prm-cre)70Og, which expresses Cre recombinase under control of the male-germline specific protamine promoter. Cre mediated excision subsequently removed the neomycin cassette upon transmission from male chimeric mice. RT-PCR indicated that no full length transcripts were produced but transcripts were produced from exons 1-6. (J:88090)

Disease models

Expression

In Structures Affected by this Mutation:

16 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Dph1^tm2Bhr

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dph1 Mutation: 27 strains or lines available

References

• Original: J:88090 Chen CM, et al., Ovca1 regulates cell proliferation, embryonic development, and tumorigenesis. Genes Dev. 2004 Feb 1;18(3):320-32
• All: 2 reference(s)