Slc4a2^tm1Jmed
Targeted Allele Detail

Summary

• Symbol: Slc4a2^tm1Jmed
• Name: solute carrier family 4 (anion exchanger), member 2; targeted mutation 1, Juan F Medina
• MGI ID: MGI:3033521
• Synonyms: Ae2^-, Ae2a,b^-
• Gene: Slc4a2 Location: Chr5:24628834-24645945 bp, + strand Genetic Position: Chr5, 11.74 cM
• Alliance: Slc4a2^tm1Jmed page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88196
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: Targeted disruption precluded the expression of 3 isoforms (Ae2a, Ae2b1, and Ae2b2) produced by the wild-type allele. A 1.5 kb genomic fragment encompassing exons 2, 1b2, and 1b1 was deleted in the germline using a cre-loxP system. The deleted exons each served as the initial coding exons for the Ae2a, Ae2b1, and Ae2b2 isoforms. The absence of transcript encoding these isoforms was verifed by RT-PCR analysis of homozygous mutant testes RNA. (J:88196)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc4a2 Mutation: 53 strains or lines available

References

• Original: J:88196 Medina JF, et al., Anion exchanger 2 is essential for spermiogenesis in mice. Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15847-52
• All: 10 reference(s)