Qki^tm1Imeg
Targeted Allele Detail

Summary

• Symbol: Qki^tm1Imeg
• Name: quaking, KH domain containing RNA binding; targeted mutation 1, Institute of Molecular Embryology and Genetics
• MGI ID: MGI:3033861
• Synonyms: qkI^-
• Gene: Qki Location: Chr17:10425480-10538706 bp, - strand Genetic Position: Chr17, 7.75 cM
• Alliance: Qki^tm1Imeg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88215
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of intron 1 and the first part of the coding sequence in exon 2, which includes the initiation sequence and the codons for the first 44 amino acids, were replaced with an IRES and a betageo fusion gene. This removes the highly conserved QUA1 domain in the encoded protein. Absence of transcription of the endogenous gene was demonstrated by RT-PCR. Beta-galactosidase is expressed from this allele. (J:88215)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Qki Mutation: 28 strains or lines available

References

• Original: J:88215 Li Z, et al., Defective smooth muscle development in qkI-deficient mice. Dev Growth Differ. 2003 Oct-Dec;45(5-6):449-62
• All: 2 reference(s)