Hsf1^tm1Miv
Targeted Allele Detail

Summary

• Symbol: Hsf1^tm1Miv
• Name: heat shock factor 1; targeted mutation 1, Nahid F Mivechi
• MGI ID: MGI:3033933
• Synonyms: hsf1^-
• Gene: Hsf1 Location: Chr15:76361562-76385355 bp, + strand Genetic Position: Chr15, 35.95 cM
• Alliance: Hsf1^tm1Miv page

Testes are smaller in Hsf2^tm1Miv/Hsf2^tm1Miv and Hsf1^tm1Miv/Hsf1^tm1Miv Hsf2^tm1Miv/Hsf2^tm1Miv mice

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88534
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 55 bp segment of exon 2 was replaced with a cassette containing IRES, lacZ, and a floxed PGK-neomycin resistance gene. The DNA binding domain was disrupted and a frame shift was created in the process. A shorter mRNA transcript in which exon 1 is spliced directly to exon 3 was detected by Northern blot analysis. Immunoblot analysis indicated the absence of any protein product. Beta-galactosidase expression was not detected. (J:88534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hsf1 Mutation: 25 strains or lines available

References

• Original: J:88534 Zhang Y, et al., Targeted disruption of hsf1 leads to lack of thermotolerance and defines tissue-specific regulation for stress-inducible Hsp molecular chaperones. J Cell Biochem. 2002;86(2):376-93
• All: 6 reference(s)