Nfkbie^tm1Syme
Targeted Allele Detail

Summary

• Symbol: Nfkbie^tm1Syme
• Name: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon; targeted mutation 1, Sylvie Memet
• MGI ID: MGI:3034029
• Synonyms: IkappaBepsilon^-
• Gene: Nfkbie Location: Chr17:45866629-45874095 bp, + strand Genetic Position: Chr17, 22.58 cM, cytoband C
• Alliance: Nfkbie^tm1Syme page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88538
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region was replaced with a lacZ gene and associated nuclear localization sequence followed by IRES and promoterless neomycin gene. Northern blot analysis established the absence of transcripts while Western blot analysis demonstrated that no protein product was present. Beta-galactosidase expression was detected in the medulla of the lymph node, and a few cells of the thymus and spleen. (J:88538)

Expression

In Mice Carrying this Mutation:	14 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfkbie Mutation: 21 strains or lines available

References

• Original: J:88538 Memet S, et al., IkappaBepsilon-deficient mice: reduction of one T cell precursor subspecies and enhanced Ig isotype switching and cytokine synthesis. J Immunol. 1999 Dec 1;163(11):5994-6005
• All: 4 reference(s)