Lmo4^tm2Sho
Targeted Allele Detail

Summary

• Symbol: Lmo4^tm2Sho
• Name: LIM domain only 4; targeted mutation 2, Stuart Orkin
• MGI ID: MGI:3035914
• Synonyms: Lmo4^-
• Gene: Lmo4 Location: Chr3:143894291-143910981 bp, - strand Genetic Position: Chr3, 68.61 cM
• Alliance: Lmo4^tm2Sho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88168
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A floxed cassette containing both a PGK-neomycin gene and a cytosine deaminase gene was inserted 5' of exon 2 while a third loxP site was inserted 3' of the exon. Chimeric mice were crossed with cre expressing transgenic mice leading to the excision of both the floxed neomycin and cytosine deaminase genes as well as exon 2. Western blot analysis confirmed the absence of protein product. (J:88168)

Expression

In Mice Carrying this Mutation:	18 assay results
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lmo4 Mutation: 9 strains or lines available

References

• Original: J:88168 Hahm K, et al., Defective neural tube closure and anteroposterior patterning in mice lacking the LIM protein LMO4 or its interacting partner Deaf-1. Mol Cell Biol. 2004 Mar;24(5):2074-82
• All: 4 reference(s)