Pas19^A/J
QTL Variant Detail

Summary

• QTL variant: Pas19^A/J
• Name: pulmonary adenoma susceptibility 19; A/J
• MGI ID: MGI:3036102
• QTL: Pas19 Location: Chr17:29711164-34754437 bp Genetic Position: Chr17, Syntenic

Variant origin

• Strain of Specimen: A/J

Variant description

• Allele Type: QTL

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:83970

Previously identified QTLs Pas1 and Pas2 were confirmed and fine mapped using (A/J x C57BL/6) advanced intercross lines (AIL) at the F11 generation. Parental strain A/J is susceptible to lung tumors where as parental strain C57BL/6 is resistant.

Pas1 was resolved to a 1 cM - 1.3 cM interval on mouse Chromosome 6 near Kras with a LOD score > 150 at D6Mit57 (71.7 cM).

1.7.2016 - Curator Note: Because Pas1 was orginally mapped in J:17778, in 1997 using an (C57BL/6J x A/J)F2 intercross, which differs in direction from the cross used here, we consider the current study a separate mapping experiment and have named this QTL Pas15 .

Candidate genes in the region of Pas15 include Lrmp, 4933403M22Rik, 4930469P12Rik, Bcat1 (Eca39), 5133400D11Rik (mHoj-1), and Sspn (Krag). SNP analysis of these candidate genes revealed coding region polymorphisms in Lrmp, 4933403M22Rik, and 4930469P12Rik, but not in Bcat1 (Eca39), 5133400D11Rik (mHoj-1), or Sspn (Krag). Candidate gene 4933403M22Rik is highly associated with Pas15 and is referred to by the authors as Pas1c1 (Pas1 candidate gene 1). Semi-quantitative RT-PCR analysis of 4933403M22Rik revealed the presence of different isoform transcripts in C57BL/6 and A/J.

Pas2 was localized to a 7.6 Mb region on mouse Chromosome 17 between D17Mit23 and D17Mit231 with the strongest association (LOD=3.0) at D17Mit16 (17.4 cM). There may be a possible interaction between Pas1 and Pas2.

1.4.2016 - Curator Note: Because Pas2 was originally mapped in J:21801 using (C57BL/6JOlaHsd x A/JOlaHsd)F2 intercross and backcross populations, which differ in direction from the cross used here, we consider the current study a separate mapping experiment and have named the QTL, Pas19.

Pas3 was not detected in this study using advanced intercross lines.

References

• Original: J:83970 Wang M, et al., Fine mapping and identification of candidate pulmonary adenoma susceptibility 1 genes using advanced intercross lines. Cancer Res. 2003 Jun 15;63(12):3317-24
• All: 1 reference(s)