Acly^{Gt(NPX098)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Acly^{Gt(NPX098)Byg}
• Name: ATP citrate lyase; gene trap NPX098, BayGenomics
• MGI ID: MGI:3036686
• Synonyms: Acly^-, Acly^{Gt(pGT1TMpfs)1Abe}
• Gene: Acly Location: Chr11:100367179-100418826 bp, - strand Genetic Position: Chr11, 63.47 cM
• Alliance: Acly^{Gt(NPX098)Byg} page

Mutation origin

• Mutant Cell Line: NPX098
• Germline Transmission: Earliest citation of germline transmission: J:88490
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The gene was disrupted by the random integration of the pGT1TMpfs gene trap vector into intron 1. The mutation results in the production of a fusion transcript consisting of sequences from exon 1 of Acly and Beta-geo. In heterozygotes, northern and western blot analyses showed an approximately 50% reduction in mRNA and protein expression. (J:88490)

Expression

In Mice Carrying this Mutation:

70 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Acly Mutation: 66 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

mutant ES cell line = NPX098 (Bay Genomics). Bay Genomics has withdrawn this line from public access, according to their web site.

References

• Original: J:88490 Beigneux AP, et al., ATP-citrate lyase deficiency in the mouse. J Biol Chem. 2004 Mar 5;279(10):9557-64
• All: 1 reference(s)