Gata1^tm6Sho
Targeted Allele Detail

Summary

• Symbol: Gata1^tm6Sho
• Name: GATA binding protein 1; targeted mutation 6, Stuart Orkin
• MGI ID: MGI:3037864
• Synonyms: dblGATA1, deltadbl GATA, deltadblGATA, Eos^-, Gata1^tm5Sho
• Gene: Gata1 Location: ChrX:7825504-7842844 bp, - strand Genetic Position: ChrX, 3.59 cM, cytoband A2
• Alliance: Gata1^tm6Sho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88711
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A high-affinity, palindromic "double" GATA protein binding site in the Gata1 promoter presumed to mediate positive Gata1 autoregulation was replaced by a floxed Pgk-neo cassette; transient Cre recombinase expression in ES cells left a single loxP site flanked by two Not1 sites. The 21-bp deleted segment comprised nucleotides -691 through -671 upstream of the last nucleotide of the first hematopoietically expressed exon of Gata1. (J:88711)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Gata1 Mutation: 45 strains or lines available

References

• Original: J:88711 Yu C, et al., Targeted deletion of a high-affinity GATA-binding site in the GATA-1 promoter leads to selective loss of the eosinophil lineage in vivo. J Exp Med. 2002 Jun 3;195(11):1387-95
• All: 110 reference(s)