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Ebf3tm1Reed
Targeted Allele Detail
Summary
Symbol: Ebf3tm1Reed
Name: early B cell factor 3; targeted mutation 1, Randall R Reed
MGI ID: MGI:3037961
Synonyms: O/E2lacz
Gene: Ebf3  Location: Chr7:136795402-136916174 bp, - strand  Genetic Position: Chr7, 82.26 cM, cytoband F3-F4
Alliance: Ebf3tm1Reed page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:88585
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA cassette containing a tau-lacZpA reporter with a floxed tk-neo component was inserted in the place of exons 1-6 by homologous recombination, and remained under transcriptional control of the endogenous promotor. The exons encoded the first 185 amino acids, which contain domains crucial for DNA binding and transactivation of the protein binding site. Subsequent cre-mediated recombination removed the tk-neo cassette. In situ hybridization indicated lack of endogenous gene expression in homozygous mutants. The expression pattern of lacZ recapitulated that of the endogenous gene. (J:88585)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ebf3 Mutation:  38 strains or lines available
References
Original:  J:88585 Wang SS, et al., Genetic disruptions of O/E2 and O/E3 genes reveal involvement in olfactory receptor neuron projection. Development. 2004 Mar;131(6):1377-88
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory