Ebf2^tm1Reed
Targeted Allele Detail

Summary

• Symbol: Ebf2^tm1Reed
• Name: early B cell factor 2; targeted mutation 1, Randall R Reed
• MGI ID: MGI:3037977
• Synonyms: OE3/EBF2-tanGFP, O/E3^GFP
• Gene: Ebf2 Location: Chr14:67470741-67668367 bp, + strand Genetic Position: Chr14, 34.63 cM, cytoband D-E1
• Alliance: Ebf2^tm1Reed page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88585
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cassette containing tau-GFPpA reporter with a floxed tk-neo component was inserted in the place of exons 1-5 by homologous recombination, and remained under transcriptional control of the endogenous promoter. The exons encoded the first 162 amino acids, which contain domains crucial for DNA binding and transactivation of the protein binding site. Subsequent cre-mediated recombination removed the tk-neo cassette. In situ hybridization indicated lack of endogenous gene expression. The expression pattern of GFP recapitulated that of the endogenous gene. (J:88585)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ebf2 Mutation: 30 strains or lines available

References

• Original: J:88585 Wang SS, et al., Genetic disruptions of O/E2 and O/E3 genes reveal involvement in olfactory receptor neuron projection. Development. 2004 Mar;131(6):1377-88
• All: 9 reference(s)