Gata1^tm1Mym
Targeted Allele Detail

Summary

• Symbol: Gata1^tm1Mym
• Name: GATA binding protein 1; targeted mutation 1, Masayuki Yamamoto
• MGI ID: MGI:3038080
• Synonyms: GATA-1.05, GATA1.05, Gata^G1.05
• Gene: Gata1 Location: ChrX:7825504-7842844 bp, - strand Genetic Position: ChrX, 3.59 cM, cytoband A2
• Alliance: Gata1^tm1Mym page

Variable phenotypes of Gata1^tm1Mym/Gata1⁺ female embryos including anemia and reduced number of hematopoietic cells

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:40321
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: In order to weaken activity of the erythroid-specific promoter, homologous recombination was used to insert a neo cassette into the intergenic region between a GATA motif known to positively regulate transcription and the first erythroid specific exon (IE). The GATA is located approximately 620 bp 5' to IE. RT-PCR showed that gene expression in mutant male embryos was less than 5% of the level of expression in wild-type embryos. (J:40321)

Disease models

Expression

In Mice Carrying this Mutation:	25 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gata1 Mutation: 45 strains or lines available

References

• Original: J:40321 Takahashi S, et al., Arrest in primitive erythroid cell development caused by promoter-specific disruption of the GATA-1 gene. J Biol Chem. 1997 May 9;272(19):12611-5
• All: 17 reference(s)