Gad2^tm1Miya
Targeted Allele Detail

Summary

• Symbol: Gad2^tm1Miya
• Name: glutamic acid decarboxylase 2; targeted mutation 1, Jun-ichi Miyazaki
• MGI ID: MGI:3038621
• Synonyms: GAD65^-
• Gene: Gad2 Location: Chr2:22512262-22583889 bp, + strand Genetic Position: Chr2, 15.15 cM, cytoband A-B
• Alliance: Gad2^tm1Miya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88785
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: An in frame translational stop codon was inserted in exon 2 by homologous recombination. Absence of Gad2 mRNA and protein in homozygotes was confirmed by Northern and Western blot analysis of brains. (J:88785, J:89181)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gad2 Mutation: 60 strains or lines available

References

• Original: J:88785 Yamamoto T, et al., Development of autoimmune diabetes in glutamic acid decarboxylase 65 (GAD65) knockout NOD mice. Diabetologia. 2004 Feb;47(2):221-4
• All: 7 reference(s)