Il7r^tm1Iku
Targeted Allele Detail

Summary

• Symbol: Il7r^tm1Iku
• Name: interleukin 7 receptor; targeted mutation 1, Koichi Ikuta
• MGI ID: MGI:3038693
• Synonyms: 7Ralpha-, Il-7R^-
• Gene: Il7r Location: Chr15:9505874-9530262 bp, - strand Genetic Position: Chr15, 4.16 cM
• Alliance: Il7r^tm1Iku page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:34658
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Homologous recombination was used to replace exon 2, containing a conserved cysteine motif, with a neo cassette. RT-PCR revealed a shorter transcript in homozygous mutants than that of wild-type mice, putatively lacking exon 2 and resulting in a frame shift mutation. Flow cytometry indicated an absence of protein surface expression on homozygous spleen cells. (J:34658)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Il7r Mutation: 31 strains or lines available

References

• Original: J:34658 Maki K, et al., Interleukin 7 receptor-deficient mice lack gammadelta T cells. Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):7172-7
• All: 15 reference(s)