Tnfsf13^tm1Geha
Targeted Allele Detail

Summary

• Symbol: Tnfsf13^tm1Geha
• Name: tumor necrosis factor (ligand) superfamily, member 13; targeted mutation 1, Raif S Geha
• MGI ID: MGI:3038886
• Synonyms: APRIL^-
• Gene: Tnfsf13 Location: Chr11:69573672-69576610 bp, - strand Genetic Position: Chr11, 42.86 cM, cytoband B4
• Alliance: Tnfsf13^tm1Geha page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88910
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A portion of of exon 3 and all of exons 4 through 6 were replaced by homologous recombination with a neo cassette in the opposite orientation of the endogenous gene. These exons encode the last 126 amino acids of the 201 amino acid long extracellular domain. RT-PCR of spleen and bone marrow indicated no transcript was present in mutant mice; in contrast, mRNA expression of Tnfsf12 and Senp3 (which flank within ~1 kb) in spleen was normal. (J:88910)

Expression

In Mice Carrying this Mutation:	9 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnfsf13 Mutation: 70 strains or lines available

References

• Original: J:88910 Castigli E, et al., Impaired IgA class switching in APRIL-deficient mice. Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3903-8
• All: 5 reference(s)