Btg2^tm1Wbh
Targeted Allele Detail

Summary

• Symbol: Btg2^tm1Wbh
• Name: BTG anti-proliferation factor 2; targeted mutation 1, Weiland B Huttner
• MGI ID: MGI:3038949
• Synonyms: Tis21-EGFP, Tis21-GFP, Tis21^tm2(Gfp)Wbh
• Gene: Btg2 Location: Chr1:134002908-134006858 bp, - strand Genetic Position: Chr1, 58.1 cM
• Alliance: Btg2^tm1Wbh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88653
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Homologous recombination was used to replace the coding portion of exon 1 with GFPpA fused to a floxed neo cassette. Subsequent cre-mediated recombination removed the neo cassette, leaving GFPpA and one loxp site. The level of GFP fluorescence in developing CNS of heterozygous mice E9.5-E14.5 matched known expression of the endogenous protein. (J:88653)

Expression

In Mice Carrying this Mutation:

32 assay results 2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Btg2 Mutation: 16 strains or lines available

References

• Original: J:88653 Haubensak W, et al., Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis. Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3196-201
• All: 26 reference(s)