Ms4a1^tm1Tft
Targeted Allele Detail

Summary

• Symbol: Ms4a1^tm1Tft
• Name: membrane-spanning 4-domains, subfamily A, member 1; targeted mutation 1, Thomas F Tedder
• MGI ID: MGI:3038963
• Synonyms: CD20^-
• Gene: Ms4a1 Location: Chr19:11227043-11243513 bp, - strand Genetic Position: Chr19, 8.19 cM, cytoband B
• Alliance: Ms4a1^tm1Tft page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88933
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of exon 6 and all of exons 7-8 were replaced with a neomycin resistance gene via homologous recombination. These exons encode part of the second extracellular loop, the fourth transmembrane domain and the large C-terminal cytoplasmic domain of the endogenous protein. The resulting protein was truncated at amino acid 157 and was fused with an 88-amino-acid peptide coded for by the neo gene promoter sequence. Absence of cell-surface protein expression was verified by flow cytometry. (J:88933)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ms4a1 Mutation: 24 strains or lines available

References

• Original: J:88933 Uchida J, et al., Mouse CD20 expression and function. Int Immunol. 2004 Jan;16(1):119-29
• All: 5 reference(s)