Atp8b3^tm1Gar
Targeted Allele Detail

Summary

• Symbol: Atp8b3^tm1Gar
• Name: ATPase, class I, type 8B, member 3; targeted mutation 1, David L Garbers
• MGI ID: MGI:3039498
• Synonyms: SAPLT^-
• Gene: Atp8b3 Location: Chr10:80355418-80374958 bp, - strand Genetic Position: Chr10, 39.72 cM, cytoband C1
• Alliance: Atp8b3^tm1Gar page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88796
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment encompassing exons 4 through 14 was replaced with a neomycin selection cassette. The deleted region encoded the first four transmembrane domains of the protein. No protein was detected in homozygous mutant mice by Western blot analysis of testes membrane extracts or by immunostaining of spermatozoa. (J:88796)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Atp8b3 Mutation: 60 strains or lines available

References

• Original: J:88796 Wang L, et al., A novel aminophospholipid transporter exclusively expressed in spermatozoa is required for membrane lipid asymmetry and normal fertilization. Dev Biol. 2004 Mar 1;267(1):203-15
• All: 1 reference(s)