Prm1^tm1Eddy
Targeted Allele Detail

Summary

• Symbol: Prm1^tm1Eddy
• Name: protamine 1; targeted mutation 1, Edward M Eddy
• MGI ID: MGI:3039794
• Gene: Prm1 Location: Chr16:10614196-10614687 bp, - strand Genetic Position: Chr16, 5.87 cM, cytoband cen-C3
• Alliance: Prm1^tm1Eddy page

Mutation origin

• Mouse Generated: Earliest citation of chimera generation: J:69127
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neo cassette replaced 34 bp in exon 1 via homologous recombination. Analysis of 129 derived sperm from chimeras showed reduced protein expression. (J:69127)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prm1 Mutation: 5 strains or lines available

Notes

ES cell line was either R1 or BK4. Haploinsufficiency of Prm1 prevents genetic transmission of the mutated allele and generation of non-chimeric heterozygous mice. All phenotype data reported are from chimeric mice.

References

• Original: J:69127 Cho C, et al., Haploinsufficiency of protamine-1 or -2 causes infertility in mice. Nat Genet. 2001 May;28(1):82-6
• All: 1 reference(s)