Stat3^tm2Vpo
Targeted Allele Detail

Summary

• Symbol: Stat3^tm2Vpo
• Name: signal transducer and activator of transcription 3; targeted mutation 2, Valeria Poli
• MGI ID: MGI:3040152
• Synonyms: Stat3^deltaalpha
• Gene: Stat3 Location: Chr11:100777632-100830447 bp, - strand Genetic Position: Chr11, 63.82 cM
• Alliance: Stat3^tm2Vpo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89194
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: To generate an allele in which the expression of the alpha isoform is completely ablated while the expression of the beta isoform is maintained, cDNA encoding the C-terminal of the beta isoform, as well as a polyA-neo cassette, was inserted into exon 20. RT-PCR and Western blot analyses of homozygous mutant liver confirmed the absence of the alpha isoform. Comparisions with the level of alpha isoform transcript and protein in wild-type mice indicated that while the level of beta isoform transcript is equivalent, the level of beta isoform protein is reduced. (J:89194)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stat3 Mutation: 72 strains or lines available

References

• Original: J:89194 Maritano D, et al., The STAT3 isoforms alpha and beta have unique and specific functions. Nat Immunol. 2004 Apr;5(4):401-9
• All: 1 reference(s)