Epha2^tm1Jrui
Targeted Allele Detail

Summary

• Symbol: Epha2^tm1Jrui
• Name: Eph receptor A2; targeted mutation 1, Joseph C Ruiz
• MGI ID: MGI:3040415
• Synonyms: Epha2-, Epha2^tm1Jinc
• Gene: Epha2 Location: Chr4:141028551-141056695 bp, + strand Genetic Position: Chr4, 73.67 cM, cytoband D-E
• Alliance: Epha2^tm1Jrui page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89275
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neo cassette was inserted at nucleotide 1372, within the sequence encoding the extracellular domain. Although the resultant transcript putatively encodes a nonfunctional protein truncated at amino acid 426, no protein was detected by Western blot analysis of murine pulmonary microvascular endothelial cells from mutant mice. (J:89275)

Expression

In Mice Carrying this Mutation:

2 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Epha2 Mutation: 96 strains or lines available

References

• Original: J:89275 Brantley-Sieders DM, et al., EphA2 receptor tyrosine kinase regulates endothelial cell migration and vascular assembly through phosphoinositide 3-kinase-mediated Rac1 GTPase activation. J Cell Sci. 2004 Apr 15;117(Pt 10):2037-49
• All: 28 reference(s)