Elsgp1^NZB/BlNJ
QTL Variant Detail

Summary

• QTL variant: Elsgp1^NZB/BlNJ
• Name: elevated serum gp70 1; NZB/BlNJ
• MGI ID: MGI:3040469
• QTL: Elsgp1 Location: Chr4:138342649-138342753 bp Genetic Position: Chr4, cM position of peak correlated region/allele: 70.47 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: NZB/BlNJ

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers elevated serum gp70 levels compared to C57BL/6J. (J:63674)
• Inheritance: Other (see notes)

Notes

Elsgp1 exhibits additive inhertiance.

Animals homozygous for NZB/BlNJ-derived alleles at both Elsgp1 and Elsgp2 exhibit the highest levels of serum gp70.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:63674

Linkage analysis was performed on 152 female (B6.H2^z x NZB/BlNJ)F1 x NZB/BlNJ backcross animals and 163 female (NZB/BlNJ x C57BL/6J)F2 intercross animals to identify QTLs linked to elevated serum gp70 (which is associated with the development of gp70 immune complexes and nephritis). 90 microsatellite markers were used in the genome scan. Parental strain NZB/BlNJ is susceptible to autoimmune nephritis compared to parental strain C57BL/6J.

In both the backcross and intercross populations two significant loci, Elsgp1 (elevated serum gp70 1) and Elsgp2, mapped to 66.6 cM on mouse Chromosome 4 (LOD=11.2 at D4Mit170) and to 40 cM on mouse Chromosome 13 (LOD=11 at D13Mit98), respectively. Elsgp2 maps near a previously identified QTL on chromosome 13 named Yaa1 (35 cM) that is also associated with serum gp70 levels. A third locus, Elsgp3, mapped to 49.6 cM on mouse Chromosome 2 (LOD=4.9 at D2Mit14). NZB/BlNJ-derived alleles confer elevated serum gp70 at all 3 loci with additive inheritance. Animals homozygousfor NZB/BlNJ-derived alleles at both Elsgp1 and Elsgp2 exhibit the highest levels of serum gp70. Elsgp1, Elsgp2, and Elsgp3 together account for 83% of the phenotypic variance in F2 mice. These loci, however, do not show linkage to gp70 immune complex levels.

In the backcross population a previously identified QTL on distal mouse Chromosome 1 named Nba2 was detected in linkage to autoantibody production and nephritis, but not to serum gp70 levels. Nba2 did not show linkage in the F2 population.

References

• Original: J:63674 Tucker RM, et al., Genetic control of glycoprotein 70 autoantigen production and its influence on immune complex levels and nephritis in murine lupus. J Immunol. 2000 Aug 1;165(3):1665-72
• All: 1 reference(s)