Pomc^tm1Sora
Targeted Allele Detail

Summary

• Symbol: Pomc^tm1Sora
• Name: pro-opiomelanocortin-alpha; targeted mutation 1, S O'Rahilly
• MGI ID: MGI:3040514
• Synonyms: Pomc^-
• Gene: Pomc Location: Chr12:4004951-4010642 bp, + strand Genetic Position: Chr12, 1.99 cM
• Alliance: Pomc^tm1Sora page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89244
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The majority of exon 3 was replaced with a tau-lacZ reporter and a floxed PGK-neo cassette. A point mutation was introduced into the initiator ATG to create a stop codon. X-gal staining showed beta-galactosidase activity was present in mutant brains. Endogenous mRNA was absent as shown by Northern blot, and a two-site immunoassay confirmed that no peptides were detected in mutant plasma. (J:89244)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pomc Mutation: 21 strains or lines available

References

• Original: J:89244 Challis BG, et al., Mice lacking pro-opiomelanocortin are sensitive to high-fat feeding but respond normally to the acute anorectic effects of peptide-YY(3-36). Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4695-700
• All: 12 reference(s)