Kcne1^tm1Rdn
Targeted Allele Detail

Summary

• Symbol: Kcne1^tm1Rdn
• Name: potassium voltage-gated channel, Isk-related subfamily, member 1; targeted mutation 1, Dan M Roden
• MGI ID: MGI:3040614
• Synonyms: minK^-, minK-lacZ
• Gene: Kcne1 Location: Chr16:92142889-92156356 bp, - strand Genetic Position: Chr16, 53.57 cM
• Alliance: Kcne1^tm1Rdn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:53735
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region, contained in exon 2, was replaced with an NLS-lacZ-pA PGK-neo cassette. Absence of mRNA was confirmed in mutant animals in an RNAse protection assay, and beta-galactosidase was detected in restricted areas of hearts of mutant mice. (J:53735)

Expression

In Mice Carrying this Mutation:

68 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Kcne1 Mutation: 13 strains or lines available

References

• Original: J:53735 Kupershmidt S, et al., Replacement by homologous recombination of the minK gene with lacZ reveals restriction of minK expression to the mouse cardiac conduction system. Circ Res. 1999 Feb 5;84(2):146-52
• All: 9 reference(s)