Dmp1^tm1Mis
Targeted Allele Detail

Summary

• Symbol: Dmp1^tm1Mis
• Name: dentin matrix protein 1; targeted mutation 1, Yuji Mishina
• MGI ID: MGI:3040629
• Synonyms: dmp1 KO, Dmp1^tm1Jqf
• Gene: Dmp1 Location: Chr5:104350479-104361968 bp, + strand Genetic Position: Chr5, 50.61 cM
• Alliance: Dmp1^tm1Mis page

Abnormal tooth morphology and mineralization in Dmp1^tm1Mis/Dmp1^tm1Mis pups

Show the 5 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85859
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 6 was disrupted by an IRES-lacZ reporter and floxed neo cassette that replaced sequence encoding Met119 through the end of the gene, including the RGD region. Mutant calvarial cells showed no wild-type mRNA in either Northern blot or RT-PCR. Immunostaining of osteocytes confirmed lack of wild-type protein in mutant mice, and X-gal staining demonstrated lacZ activity in heterozygote hypertrophic chondrocytes, osteoblasts and osteocytes. (J:85859)

Disease models

Expression

In Structures Affected by this Mutation:

19 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dmp1 Mutation: 42 strains or lines available

References

• Original: J:85859 Feng JQ, et al., The Dentin Matrix Protein 1 (Dmp1) is Specifically Expressed in Mineralized, but not Soft, Tissues during Development. J Dent Res. 2003 Oct;82(10):776-80
• All: 34 reference(s)