Dnaja3^tm1.2Jdl
Targeted Allele Detail

Summary

• Symbol: Dnaja3^tm1.2Jdl
• Name: DnaJ heat shock protein family (Hsp40) member A3; targeted mutation 1.2, Jiing-Dwan Lee
• MGI ID: MGI:3040718
• Synonyms: Td1^-
• Gene: Dnaja3 Location: Chr16:4501934-4525559 bp, + strand Genetic Position: Chr16, 2.46 cM
• Alliance: Dnaja3^tm1.2Jdl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89091
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Cre-mediated recombination removed the floxed exon 2 in Dnaja3^tm1Jdl, creating a frame-shift that putatively disrupted translation of the Dnaj domain, a glycine-and phenylalanine-rich region, a systein-rich part resembling a zinc finger, and a nonhomologous C-terminal domain. Western blot of mutant MEFs demonstrated that protein production was abolished. (J:89091)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnaja3 Mutation: 43 strains or lines available

References

• Original: J:89091 Lo JF, et al., Tid1, a cochaperone of the heat shock 70 protein and the mammalian counterpart of the Drosophila tumor suppressor l(2)tid, is critical for early embryonic development and cell survival. Mol Cell Biol. 2004 Mar;24(6):2226-36
• All: 1 reference(s)