Tpm3^tm1Pgun
Targeted Allele Detail

Summary

• Symbol: Tpm3^tm1Pgun
• Name: tropomyosin 3, gamma; targeted mutation 1, Peter Gunning
• MGI ID: MGI:3040795
• Gene: Tpm3 Location: Chr3:89979958-90008209 bp, + strand Genetic Position: Chr3, 39.2 cM
• Alliance: Tpm3^tm1Pgun page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89057
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A single loxP site remained in place of the first coding exon (1b) and the donor splice junction following the excision of a floxed neo cassette by transient Cre-mediated recombination. This mutation was designed to eliminate all non-muscle type isoforms of the encoded protein. Western blot showed that protein levels in heterozygote brains were the same as that of wild-type. (J:89057)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tpm3 Mutation: 48 strains or lines available

References

• Original: J:89057 Hook J, et al., Gamma tropomyosin gene products are required for embryonic development. Mol Cell Biol. 2004 Apr;24(6):2318-23
• All: 1 reference(s)