Runx1^tm4Dow
Targeted Allele Detail

Summary

• Symbol: Runx1^tm4Dow
• Name: runt related transcription factor 1; targeted mutation 1, James R Downing
• MGI ID: MGI:3040872
• Synonyms: Runx1-IRES-GFP, Runx1-IRES-GFP^k
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^tm4Dow page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89055
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: An IRES-GFP-pA-neo cassette was inserted downstream of the coding region. Flow cytometry, RT-PCR and Western blot indicated presence of GFP expression, Runx1 transcript, and protein, respectively, in mutant mice. (J:89055)

Expression

In Mice Carrying this Mutation:

3 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 34 strains or lines available

References

• Original: J:89055 Lorsbach RB, et al., Role of RUNX1 in adult hematopoiesis: analysis of RUNX1-IRES-GFP knock-in mice reveals differential lineage expression. Blood. 2004 Apr 1;103(7):2522-9
• All: 12 reference(s)