Dnase2a^tm1Aea
Targeted Allele Detail

Summary

• Symbol: Dnase2a^tm1Aea
• Name: deoxyribonuclease II alpha; targeted mutation 1, Alan Eastman
• MGI ID: MGI:3040926
• Synonyms: DNase IIalpha -
• Gene: Dnase2a Location: Chr8:85635384-85638332 bp, + strand Genetic Position: Chr8, 41.31 cM
• Alliance: Dnase2a^tm1Aea page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89469
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The first five exons were replaced with a PGK-neomycin resistance gene via homologous recombination. The genetic disruption resulted in complete loss of acid endonuclease activity in mouse embryonic fibroblasts and E15.5 livers from homozygous mutant embryos. (J:89469)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Dnase2a^tm1Aea/Dnase2a^tm1Aea	involves: 129 * C57BL/6

Phenotypes:

Affected Systems	hm1
show or hide all annotated terms
cellular	√
abnormal apoptosis	√
hematopoietic system	√
abnormal definitive hematopoiesis	√
anemia	√
mortality/aging	√
perinatal lethality, complete penetrance	√
muscle	√
diaphragmatic hernia	√
thin diaphragm muscle	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnase2a Mutation: 93 strains or lines available

References

• Original: J:89469 Krieser RJ, et al., Deoxyribonuclease IIalpha is required during the phagocytic phase of apoptosis and its loss causes perinatal lethality. Cell Death Differ. 2002 Sep;9(9):956-62
• All: 3 reference(s)