Raf1^tm1Ulb
Targeted Allele Detail

Summary

• Symbol: Raf1^tm1Ulb
• Name: v-raf-leukemia viral oncogene 1; targeted mutation 1, Catrin Pritchard
• MGI ID: MGI:3040971
• Synonyms: raf-1^-, Raf1^-
• Gene: Raf1 Location: Chr6:115595530-115653596 bp, - strand Genetic Position: Chr6, 53.62 cM, cytoband C3
• Alliance: Raf1^tm1Ulb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89619
• Parent Cell Line: E14.1a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: A neo cassette replaced exons 10-13 (residues 338-472). Not even truncated proteins were detected in homozygous mutants in Western blot, and heterozygous mice demonstrated about 50% less protein expression than wild-type levels. (J:89619)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Raf1 Mutation: 117 strains or lines available

References

• Original: J:89619 Huser M, et al., MEK kinase activity is not necessary for Raf-1 function. EMBO J. 2001 Apr 17;20(8):1940-51
• All: 4 reference(s)