Robo1^tm1Matl
Targeted Allele Detail

Summary

• Symbol: Robo1^tm1Matl
• Name: roundabout guidance receptor 1; targeted mutation 1, Marc Tessier-Lavigne
• MGI ID: MGI:3043142
• Synonyms: Robo1-, Robo1^{gt(PLAP)1Matl}
• Gene: Robo1 Location: Chr16:72105194-72842983 bp, + strand Genetic Position: Chr16, 41.08 cM
• Alliance: Robo1^tm1Matl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89780
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The third intron was disrupted by the insertion of a PLAP secretory gene trap vector containing a splice acceptor site followed by Beta-geo, an IRES, placental alkaline phosphatase, and a PGK-1 polyA tail. Some residual RNA expression is seen in RNA protection analysis, however in immunostaining analysis no protein is detected in homozygous mutant mice. (J:89780)

Expression

In Mice Carrying this Mutation:	25 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Robo1 Mutation: 87 strains or lines available

References

• Original: J:89780 Long H, et al., Conserved roles for Slit and Robo proteins in midline commissural axon guidance. Neuron. 2004 Apr 22;42(2):213-23
• All: 67 reference(s)